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mos negative selection marker pma122—phsp-16.4  (Addgene inc)


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    Addgene inc mos negative selection marker pma122—phsp-16.4
    Mos Negative Selection Marker Pma122—Phsp 16.4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mos+negative+selection+marker+pma122+phsp/pMA122+-+peel-1+negative+selection+(Plasmid+%2334873)/pmc09020716-29-0-9
    Average 93 stars, based on 41 article reviews
    mos negative selection marker pma122—phsp-16.4 - by Bioz Stars, 2026-10
    93/100 stars

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    Sequencing:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Recombinant:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    CRISPR:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Plasmid Preparation:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Expressing:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Marker:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Selection:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..

    Software:

    Article Title: An acentriolar centrosome at the C. elegans ciliary base.
    Article Snippet: 20 OD2115 C. elegans: Strain OD2116: ltSi569[pOD1110; CEOP3608 tbg-1::mCherry; cb-unc-119(+)] I; ltSi542[pOD1506; Pptrn-1::ptrn-1cDNA::superfoldergfp; cb-unc-119(+)] II; unc-119(ed3) III 20 OD2116 C. elegans: Strain OD2509: gip-2(lt19[gip-2::gfp +loxP]) I; unc-119(ed3) III 20 OD2509 C. elegans: Strain PHX3504: pcmd-1(syb3504[mCherry::pcmd-1]) I SunyBiotech PHX3504 C. elegans: Strain TMD119: pcmd-1(syb486[gfp::pcmd-1]) I 7 TMD119 Oligonucleotides ptr-2(C32E8.8) dsRNA: 1.7mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTGTGCCCGATCATTCTGCAT 2 AD1168 ptr-2 reverse primer: AATTAACCCTCACTAAAGGGTGGCCATCCAAGAGCTGAT 2 AD1169 spd-2(F32H2.3) dsRNA: 5.4mg/mL, amplified from N2 cDNA; forward primer: AATTAACCCTCACTAAAGGGATGTGGGGTAATGGTGGAATT 2 AD60 spd-2 reverse primer: TAATACGACTCACTATAGGCGAAAATCTTGTATTGGGAACA 2 AD61 spd-5(F56A3.4) dsRNA: 2.5mg/mL, amplified from N2 genomic DNA; forward primer: TAATACGACTCACTATAGGTCGTGCTCAAGCTTGCTACA This study AD1088 spd-5 reverse primer: AATTAACCCTCACTAAAGGCCGAAGACAATTTTGCCAGACA This study AD1089 spd-5 CRISPR crRNA (target sequence: GUUUCCAUAGUAA CAAAAGU) This study N/A spd-5 CRISPR repair template: CGTTCTGCTCCCCTTTTCGTGGGGCCTCAGAAAAACGCGC GCCTAAACGTGAATTTTGAATTTTCTCCGCGTCCGCGAAAT TCTTACTCTCGAGATCATATAAAAGTTGGTCCGCACTTGTTT TTCATGATTATGCACGCCCAAAGAACGGTGCTGAAGCTTCA AATTTTGCAACCTCCTGTTCATTTTGACTCAAAACT This study N/A (Continued on next page) Current Biology 31, 1–11.e1–e8, June 7, 2021 e4 Report .. REAGENT or RESOURCE SOURCE IDENTIFIER dpy-10 co-CRISPR crRNA (target sequence: GCUACCAUAG GCACCACGAG) 55 N/A dpy-10 co-CRISPR repair template: CACTTGAACTTCAATACGGCAAGATGAGAATGACTGGAAAC CGTACCGCATGCGGTGCCTATGGTAGCGGAGCTT CACAT GGCTTCAGACCAACAGCCTAT 55 N/A tracrRNA: AACAGCAUAGCAAGUUAAAAUAAGGCUAGUCCGU UAUCAACUUGAAAAAGUGGCACCGAGUCGGUGCUUUUUUU Dharmacon Cat# U-002005-200 Recombinant DNA CRISPR repair template for GFP-AIR-1 (1.5kb flanking sequences) in pUC57 vector This study pAD609 air-1 targeting sgRNA under U6 promoter (target sequence: GCCTTCTGATCGTCTATCAC) in pUC57 vector This study pAD610 Cas9-SV40 NLS expression vector Peft-3::cas9-SV40_NLS::tbb-2utr 56 Addgene Plasmid # 46168 Mos Co-injection marker pCFJ104 - Pmyo-3::mCherry::unc-54 57 Addgene Plasmid # 19328 Mos Co-injection marker pGH8 - pRAB-3::mCherry::unc-54utr 57 Addgene Plasmid # 19359 Mos Co-injection marker pCFJ90 - Pmyo-2::mCherry::unc-54utr 57 Addgene Plasmid # 19327 Mos Negative selection marker pMA122 - Phsp-16.41::peel-1::tbb-2utr 58 Addgene Plasmid # 34873 Software and Algorithms ImageJ version 2.0.0 NIH https://imagej.nih.gov/ij/ MetaMorph 7.7.5 Molecular Devices N/A GraphPad Prism 6 GraphPad N/A Python 3.8.5 N/A https://www.python.org Sci-kit image package 0.17.1 N/A https://scikit-image.org Report ..



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